2.3.3 Notes on fluorescence analysis
(1) Raman
Scattering
When fluorescence is measured, two additional peaks may
appear in the spectrum. The Rayleigh peak appears at the
excitation wavelength and is due to scattered light, while
the Raman peak appears at longer wavelength than the
excitation. The position of the Raman band is dependent
on the excitation wavelength, while the position of the
fluorescence is independent of the excitation wavelength
though its peak height changes. Both Rayleigh and
Raman scatterings occur due to a solvent. Take care not
to mistake them for fluorescence peaks.
Table 3-2 presents the position of the Raman peak for a
variety of excitation wavelengths.
In liquid chromatography, the Raman scattering appears as
a background rise. This means that baseline noise
increases to disturb high-sensitivity measurement.
Table 2-5
Raman Spectral Peak Position at Each Excitation Wavelength
(Excitation
Wavelength)
Water
Ethanol
Cyclohexane
Carbon
Tetrachloride
Chloroform
248 271
267 267
—
—
313 350
344 344
320
346
365 416
405 408
375
410
405 469
459 458
418
461
Excitation
wavelength, and
Raman peak
position (nm)
436 511
500 499
450
502
2 - 11
Содержание CHROMASTER 5160
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