Hitachi CHROMASTER 5160, Instruction Manual

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2.3 Description of Fluorometry
2.3.1 Principle of fluorometry
Excitation
Stable condition
(Ground state)
Fluorescence or
Phosphorescence
Unstable condition
(Excited state)
Radiationless
transition
Fig. 2-4 Typical Organic Molecular Energy Level Diagram
Figure 3-4 shows the energy level transitions of an organic
molecule for the various processes involved in absorption,
fluorescence and phosphorescence.
Radiationless
transition
Absorption
Excitation light
Excited triplet
state
Excited state V =
Phosphorescence
Ground state V =
3
0
1
2
Light Light
3
Fluorescence
0
1
2
2 - 6