Hitachi CHROMASTER 5160, Instruction Manual

Page: 497 / 1022

3.4.5 Spectrum measurement
Set the wavelength scan range (maximum and minimum values)
for excitation (Ex) and emission (Em), then measure and record
the spectral data for each.
*
Carry out recording of spectral data with sample contained in
the cell (sample not flowing, but stationary in cell).
NOTE
: The spectral bandwidth is 15 nm on both excitation and
emission sides (bottom width of peak is about 30 nm).
A component with a difference between excitation and
emission wavelengths of less than 30 nm cannot be
accurately measured. Excitation and emission
wavelengths should be kept more than 30 nm apart for
measurement.
NOTE
: The maximum wavelength scan range (from end to
start wavelength) for both excitation and emission is
400 nm.
For spectrum acquisition in a range greater than
400 nm, the range should be divided into two for
measurement.
NOTE
: Saved spectral data will not be retained when the
power is turned OFF.
(1) Measurement and Recording of Spectrum for Excitation
(Ex) Scan Range:
(a) Press the SPECT key.
(b) The scan mode setting screen will appear.
<Scan mode setting screen>
SPECTRUM MODE (FL (Ex)=1, FL (Em)=2)
1
Press numeric key 1 and then the ENT to select FL
(Ex).
Now proceed to the scan range setting screen for
setting a scan range, and enter a start wavelength (a
cursor will appear at WL1).
(c) Enter a start wavelength (minimum value) at WL1 of
the scan range.
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