Hitachi CHROMASTER 5160, Instruction Manual

Page: 350 / 1022

4.2.3
4.2.3 Measurement of Baseline Drift
Detector specifications are to be checked by measuring drift of
the baseline. For this measurement, the following devices
should be prepared.
•
Recorder or Model D-2500 chromato-integrator
•
Vernier caliper (max. measurable length 200 mm or more,
minimum readable value 0.1 mm or less)
NOTE : When the inside of flow cell is wetted with liquid,
baseline noise and drift may increase.
To prevent this, wash the flow cell with an organic
solvent such as methanol, and then conduct
purging adequately using nitrogen gas. While an
organic solvent such as methanol or acetonitrile is
delivered through the cell, the baseline noise and
drift may be reduced.
Conditions
•
Wavelength : 250 nm
•
Time constant (response) : 2 sec
•
Sampling period of chromato-integrator : 1600 ms
•
Measurement time : 1 hour
•
Warm-up : 1 hour
(1) After turning on power supply, set wavelength at 250 nm
and conduct warm-up for about 1 hour.
WL
Press key.
250
WAVELENGTH (190 – 600) nm
Press [numerical] keys [2][5][0], and then the [ENT] key.
(2) Carry out auto zero.
Press key.
A/Z
0.0 250 0.0000 1 D2
TIME WL ABS PROG LAMP [I]
4 - 5