Hitachi CHROMASTER 5160, Instruction Manual

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(4) Each component separated from the sample is then
detected as a signal by the detector.
1.2 Low-Pressure Gradient System
In the low-pressure gradient system, two or more solvents are
mixed in its low-pressure section from which a liquid feed is
performed by a single pump. The composition of an eluent is
made to vary with time in chromatographic separation.
Fig. 1-2 Low Gradient System
Mobile phase
Wash fluid
Organizer
Chromaster 5110
Pump
Degassing
unit
Mixer
Proportioning valve
4-way joint
Chromaster 5440
Fluorescence detector
Chromaster 5310
Column oven
Drain
Syringe valve
Injection valve
Wash pump
Detector cell
Colum
n
Chromaster 5210
Autosampler
(1) Each of plural eluents is fed from the degassing unit to the
low-pressure unit.
(2) In the low-pressure unit, the eluents are mixed at a pre-
specified ratio.
(3) Through the mixer, the mixture eluent is delivered.
(4) A sample is injected by the autosampler.
(5) The injected sample is separated through the column which
is maintained at a constant temperature by the column oven.
(6) Each component separated from the sample is detected as
a signal by the detector.
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