Hitachi CHROMASTER 5160, Instruction Manual

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0
5
10
15
20
25
30
35
40
100 120 140 160 180 200
Loop Capacity [µl]
S
am
ple
Inj
ect
io
n
Vo
lum
e
[
µl]
Fig. 2-8 All Volume Injection Method
Full Loop Injection Method
When applying Full loop injection method, the sample
introduction volume more than quintuple loop capacity
(injection volume) is required (amount of sample
necessity).
Select sample introduction volume within the area
indicated in the following figure.
The sample introduction volume is selected within the
shadow area of the following figure to fill a loop with the
density of the 99% of the sample. (For example, more
than 25 µL sample is required when injecting 5 µL
sample (5 µL loop capacity). This means that quintuple
sample volume is required.)
5
10
15
20
25 50 75 100
Amount of sample necessity introduction [µl]
S
am
ple I
nj
ect
io
n V
o
lum
e
[
µl]
(L
oop
Cap
acit
y)
Fig. 2-9 Full Loop Injection Method
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