Hitachi CHROMASTER 5160, Instruction Manual

Page: 761 / 1022

3.2 Operating Procedure
Table 3-1 Typical Solvents Restricted for Use as
Mobile Phase
No. Solvent Remarks
1 Hexafluoroisopropanol (HPIF)
2 60% hexane If replacement of hexane with other solvent in the same
chamber is repeated, the chamber might be damaged.
3 Hydrogen fluoride solvents
4 Perfluoro solvents
5 CFC solvents
6 Acid A high concentration of 1 mol/L or more will corrode the
chamber.
7 Alkali A high concentration of 1 mol/L or more will corrode the
chamber.
8 Salt After flowing a high-concentration buffer solution or salt-
containing solvent, be sure to wash with distilled water.
Otherwise, the instrument may be damaged due to
formation of crystals in the flow path.
9 Buffer solution After flowing a high-concentration buffer solution or salt-
containing solvent, be sure to wash with distilled water.
Otherwise, the instrument may be damaged due to
formation of crystals in the flow path.
3.2 Operating Procedure
(1) Turning on Power Supply
After turning on the power switch, the vacuum pump in the
instrument starts. Immediately after turning on the power
supply, the monitor LED blinks in orange. The monitor
LED (Green) lights to indicate that the degassed liquid can
be supplied.
(2) Start of Degassing
For stabilization and solvent replacement (substitution) of
the flow paths, the pump starts to begin online degassing.
If a solvent is flowed at an excessively high flow rate,
bubbles might be formed. To prevent this, the flow rate
should be within 5 mL/min. (However, the flow rate should
be within 3 mL/min for flowing 2 or more solvents in
gradient elution using the same solvent, because bubbling
is apt to occur.)
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