Hitachi CHROMASTER 5160, Instruction Manual

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Chromaster 5440
FL Detector
Chromaster 5110
Pump
Waste
solution
Distilled water
Flow cell
D-2500 Chromato-data processor
Fig. 4-1 Tubing Diagram
(2) Measurement of Raman Spectrum of Water
Set the flow cell unit in place, measure the Raman
spectrum of water under the conditions given below, and
record the results (see sections 3.4.2, 3.4.3, 3.4.5 and
3.4.6). Measure in the following sequence.
•
Excitation (Ex) wavelength : 350 nm
•
Time constant (response) : 2 seconds
•
Photomultiplier voltage : MID
•
Emission spectrum
bandwidth : STANDARD (15 nm) or
WIDE (30 nm)
•
Emission spectrum
measuring wavelength : 350 to 450 nm
•
Spectrum No. : Any of 1 to 4
(a) Turn ON the power switch and the 1
st
monitor screen
appears.
(b) Set an excitation wavelength.
(i) Press the
WL
key and the wavelength setting
screen appears. Set an excitation (Ex) wavelength.
4 - 3