Hitachi CHROMASTER 5160, Instruction Manual

Page: 452 / 1022

80
60
Relati
ve intensity (%)
40
Raman scattering
20
0
350 400 (nm)
Excitation wavelength
Fig. 2-7 Raman Spectrum of Water
(2) Notes on Handling Samples that Contain a High
Concentration of the Compound of Interest
When the concentration of the compound of interest is
relatively high, various error factors will arise.
The greatest factor is that the excitation beam is
substantially absorbed near the entrance slit of a cell and it
cannot reach the cell center adequately.
An extreme situation is shown in Fig. 3-8. Fluorescence is
emitted near the entrance slit of a cell, but it will not be
taken into the emission monochromator.
Excitation beam
Fluorescence emitted
Fluorescence
Fig. 2-8 Sample Having Extremely High Concentration
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