Hitachi CHROMASTER 5160, Instruction Manual

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(g) Shut off the city water.
(h) Turn off the power of ultrasonic cleaner.
(i) Take mobile phase container out of ultrasonic cleaner,
wipe the water from the container, and set it to liquid
chromatograph.
NOTE
: Bubble generation depends on constitution of
mobile phase.
When mobile phase is a mixture with organic
solvent (e.g. methanol 20% + water 80%), fix
degassing time because constitution ratio of
mobile phase may be varied by degassing time
(boiling point of mobile phase would vary with a
reduced pressure).
3.7.3 Removing dust from mobile phase
There are various ways to remove dust from mobile phase.
Remove dust in the way suitable for the purpose of use.
(1) Example of Filtering of Mobile Phase by Using Membrane
Filter
Removing dust and degassing can be performed
simultaneously by filtration with a 0.45
μ
m membrane filter.
Membrane filter
NOTE
: Remove dust from mobile phase by using a
0.45
μ
m membrane filter. (Do not use filter paper
because it generates dust.)
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