Hitachi CHROMASTER 5160, Instruction Manual

Page: 551 / 1022

Connect the syringe to the tip of the drain tube, and inject
the wash fluid into the cell for cleaning (Fig. 5-7).
NOTE
: This flow cell unit (P/N 890-2967) uses a part similar to
the one used in the Chromaster 5440 FL Detector.
Be careful not to mount the part of the flow cell unit to
the detector and vice versa.
Fig. 4-9 Flow Cell Unit
(6) Replace the wash fluid with the mobile phase that is used
for separation, and repeat the procedure in step (5).
(7) If air bubbles are observed in the flow cell, supply solution
into the cell with the pump at a flow rate within 1.0 mL/min
and a pressure within 1.0 MPa. While the solution is being
supplied into the flow cell, pinch the end of the drain tube
with your fingers for a few seconds to increase the pressure
inside the cell, then release your fingers quickly.
Repeat this procedure a few more times until all the air
bubbles have been removed.
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