Hitachi CHROMASTER 5160, Instruction Manual

Page: 450 / 1022

In contrast, if the fluorescence spectra do not overlap (Fig.
3-6 (b)), quantitative analysis of each compound can be made by
selecting the appropriate fluorescence emission wavelength
(even if the absorption wavelengths of two components are the
same).
Absorption spectra
Component A
Component B
Excitation spectra
Component A
Fluorescence spectra
Component B
(a) Absorptiometry (b) Fluorometry
Fig. 2-6 Spectroscopic Measurement of a Sample
Containing Two Components
Table 3-1 compares the analytical information obtained via
absorptiometry and fluorometry.
Table 2-4 Comparison of Analytical Information
Attainable with Absorptiometry and Fluorometry
Absorptiometry Fluorometry
Absorption spectra only
(corresponding to excitation spectra)
•
Excitation spectra
•
Fluorescence spectra
2 - 10