Hitachi CHROMASTER 5160, Instruction Manual

Page: 919 / 1022

3.6 Precautions on Handling
(1) Solvent Displacement and Washing in Flow Path
For displacement in the flow path, mutually soluble solvents
should be used. (Be sure to perform solvent displacement
in the detector cell and sample injector flow path also.)
For washing in the flow path, take the same procedure.
If a rapid replacement is made, salt
precipitation may occur to cause clogging.
Polar organic
solvent
Buffer solution Distilled water Organic solvent
(e.g. salt solution)
(e.g. hexane,
isopropyl ether)
(e.g. methanol,
ethanol)
Shown above is an example of displacement/washing for
changeover from amino acid analysis to liposoluble vitamin
analysis.
(2) Storage of Column
When leaving the column unused for a long time, fill it with
a solvent and seal its outlet and inlet.
(a) Run a solvent through the column continuously (at a
flow rate of 0.2 mL/min; 0.02 mL/min for a semi-micro
type of column)
(b) Remove the outlet tubing, and attach the sealing plug
to outlet.
(c) Stop the pump.
(d) Remove the inlet tubing, and attach the sealing plug to
the inlet.
(e) Using a wrench, secure the sealing plugs attached at
both ends of the column.
* Before removal, feed an easy-to-elute solvent and
then make displacement with a certain solvent.
(3) Selection of Solvent
(a) Check the absorption characteristic (wavelength) of a
(Outlet)
Plug
Pump
0.2 mL/min
(0.02 mL/min)
(Inlet)
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