(4) Wash of the needle outer wall with the wash fluid1
The syringe aspirates one-half of the air volume (:va/2) while
needle is moving to the wash port.
Deliver the wash fluid1 via the wash pump to wash the needle
outer wall.
Buffer tube
vd :Dead volume (the instrument inside parameter)
vi :Injection volume (the sample net volume to the flow path)
vw :Waste volume (the air volume of the lead and rear part of a sample)
va :Air volume (the air volume which sample is sandwiched.)
Injection valve
Syringe valve
Syringe
Wash pump
5
1
3
2
4
Plunger
wash
Injection port
5
4
3
2
1
6
Waste
solution
Loop
Wash port
Sample
Rack
P
Needle
va/2
Wash
fluid2
Wash
fluid1
Waste
solution
From Pump To Column
Degassing
unit
va/2
3-way solenoid valve
2 - 39
Summary of Contents for CHROMASTER 5160
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