Hitachi CHROMASTER 5160, Instruction Manual

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(2) Wash fluid selection and use examples
The Chromaster 5210 autosampler uses two kinds of wash
fluid. Refer to the following table.
Table 2-2 Isocratic Analysis
One Fluid Wash <standard>
(for Cut injection method,
Full loop injection method)
Two Fluids Wash
(for All volume injection method,
Low carry over analysis)
Wash fluid 1 Sample solution
*1)
,
Mobile phase
*1)
Sample solution
*1)
(Strong wash)
Wash fluid 2 Aqueous solvent
*2)
Mobile phase
*1)
(Weak wash)
Table 2-3 Gradient Analysis
One Fluid Wash <standard>
(for Cut injection method,
Full loop injection method)
Two Fluid Wash
(for All volume injection method,
Low carry over analysis)
Wash fluid 1 Sample solution
*1)
, Sample solution
*1)
Gradient final solvent
*3)
(Strong wash)
Wash fluid 2 Aqueous solvent
*2)
Gradient initial solvent
*1)
(Weak wash)
*1) As a washing solution in cases where a solvent
containing a buffer solution or salt has been fed as the
mobile phase, use a mobile-phase solvent containing no
salt.
(When 20 mmol/L KH2PO4:CH3CN
＝
50:50 is used for
the mobile phase, use H2O:CH3CN
＝
50:50.)
If a solvent containing salt is used for washing, it causes
damages, such as the surface of the glass, the tip of the
syringe, and the inside of the wash pump.
Additionally, it is resulting in deterioration of performance
or shorter service life.
*2) When the aqueous solvent is used for washing of a
plunger seal for the Chromaster 5110 pump.
For example, when an organic solvent is used as wash
fluid1 in the normal phase chromatography analysis
which the cut injection method is applied, salt
precipitation in the pump plunger can’t be washed off
(Salt doesn’t dissolve). In that case, use the aqueous
solvent as wash fluid 2.
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