Hitachi CHROMASTER 5160, Instruction Manual

Page: 805 / 1022

2.3.2 Sample Injection Method
(1) Description of each method
Select a sample injection method for the Chromaster 5210
autosampler from the following three methods.
Table 2-1 Injection Methods
Cut Injection Method
(Standard)
All Volume Injection
Method
Full Loop
Injection Method
Summary
The amount of sample
withdrawn by the needle
is slightly larger than the
amount that will be
transferred from the
injection valve to the
column. The middle
part of the sample is
injected; the leading and
trailing ends are not
injected.
This method provides
good reproducibility,
however some of the
sample is wasted. The entire sample
withdrawn by the needle
is transferred to into the
sample loop and into the
column.
This method is suitable
for analysis of minute
samples since no part of
the sample is washed. The sample loop is filled
with sample for
measurement.
This method provides
the best repeatability of
sample injection volume
in analysis.
Measurement
method
Syringe Syringe Loop
Measurement
method details
Discharge rate from
Syringe
Aspiration/Discharge
rate from syringe
The summation of the
capacity of the flow
pass in the loop and
the injection valve
Reproducibility
○ △ ◎
Sample loss
△
(occurrence)
◎
(nonoccurrence)
△
(occurrence)
Carry over ○ ◎ (Low-volume
sample handling)
○
Injection time ○ ◎ ◎
The loop
exchange when
the injection
volume is
changed
◎
(unnecessity)
◎
(unnecessity)
△
(necessity)
Injection range
◎
(broad)
○
(narrow)
△
(fixity)
Commingling of
wash fluid with
flow path
◎
(nonoccurence)
△
(occurrence)
◎
(nonoccurence)
AS dead volume
as system
○ ○ ◎
The decline of
the sample
concentration
caused by
influences of
dilution (the
diffusion).
△
(90% to 98%)
△
(90% to 98%)
○
(to 99 %)
◎
:Better,
○
:Good,
△
:Acceptable
2 - 9