Hitachi CHROMASTER 5160, Instruction Manual

Page: 467 / 1022

Then, the cursor moves to Em.
(3) Set an emission wavelength (Em).
<Em wavelength setting screen>
Ex Em (250 - 900.0) nm
250 350
Press numeric keys ((2 5 0 to 9 0 0)) and then the ENT key.
* This wavelength is settable within a range from 250 to
900 nm in increments of 1 nm.
* Default value: 350 nm
* Press the ESC key or
V key to cancel the input
emission wavelength value. It returns to the 1
st
monitor
screen.
* Pressing the U key enables you to return to the
excitation wavelength setting without changing the
emission wavelength.
(4) Wavelength setting is now completed and the 1
st
monitor
screen returns.
At Ex and Em, the set values are indicated.
<1
st
monitor screen>
TIME Ex Em FL PROG LAMP [I]
0.0 250 350 0.0 1 Xe
3.1.5 Shutdown procedure
Turn the power supply of the detector off to shut it down.
The set measurement conditions are retained by the memory
even when the power is switched off. When power is turned on
again, the conditions that were in place when power was turned
off will be restored.
3.1.6 Flushing the flow cell after use
Make sure to flush the flow cell with distilled water after using
any buffers or salts in the mobile phase. Failure to carry out
flushing may lead to clogging and/or breakage of the flow cell.
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