Beckman Coulter AU2700, Руководство пользователя

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D-126
Chapter D - Software
Automated Chemistry Analyzer
AU2700 User's Guide
May, 2002
1-POINT CAL CORRECTION
OLP4064
0
Example 1: If none of multiple standard solutions has a concentration of 0
● If the single-point correction is performed
for the CONC2 standard solution, execute
the following calculation.
1. Perform the single-point correction.
ODn' = ODn x
2. Recalculate for the calibration chart.
CONC
CONC1 CONC2 CONC3
OD3'
OD3
OD2'
OD2
OD1'
OD1
OD
OD2
OD2'
0
Example 2: If any standard solution has a concentration of 0
● If correction is performed with two points
of CONC1 and another (CONC3), execute
the following calculation.
1. Use the reaction OD values of CONC1
and CONC3 (OD1' and OD3') as they are.
2. Correct each point as follows.
ODn' = a x (ODn - OD1) + b
a =
b = OD1'
CONC
CONC2 CONC3 CONC4
OD4'
OD4
OD3'
OD3
OD2'
OD2
OD1'
OD1
OD
OD3' - OD1'
OD3 - OD1
ODn : previous OD value ODn' : OD value after correction
Process calibration on the ISE:
During ISE calibration, MID, HIGH, & LOW which have a known concentration, are measured.
The relationship between the electrode potential and ion concentration at that time is obtained,
and Na, K, and Cl calibration coefficient S (slope) is calculated.
Manual calibration:
OLP4082
E
H
-E
M
'
E
L
-E
M
''
C
L
C
H
Concentration
value
(logarithm)
mmol/L
Potential
difference (mV)
C
H
C
L
E
H
-E
M
'
E
L
-E
M
''
Set a calibration using a potential difference
between the two points of known concentration.
Calibration : A known concentration of HIGH STANDARD
liquid used for calibration
: A known concentration of LOW STANDARD
liquid used for calibration
: A potential difference between HIGH
STANDARD liquid and MID liquid
: A potential difference between LOW
STANDARD liquid and MID liquid