Beckman Coulter AU2700, Руководство пользователя

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D-94
Chapter D - Software
Automated Chemistry Analyzer
AU2700 User's Guide
D-94
Chapter D - Software
Automated Chemistry Analyzer
AU2700 User's Guide
April 18, 2008
Wavelength Sec.: Select None (no secondary wavelength), 340, 380, 410, 450, 480, 520, 540,
570, 600, 660, 700, 750, 800. The OD of the secondary wavelength will be
subtracted from the OD of the primary wavelength whenever a secondary
wavelength is selected.
Method: Select END, RATE, FIXED, END1, RATE1 or FIXED 1
Reaction Slope (increasing absorbance) or - (decreasing absorbance)
Measuring point 1 Enter the primary read points P0 to P27.
Measuring point 2
Enter the secondary read point P0 to P10..
Linearity Limit A variance check for reactions with methods defined as RATE or RATE 1. The
“*” flag is generated when the check is exceeded.
No-Lag-Time Used to determine the reaction rate for methods defined as RATE or RATE 1.
No : The option is not used.
Yes : The option is used.
Pre-Dilution Rate Enter 0 (no dilution) or 5 to 100 uL. This setting allows the analyzer to pre-
dilute the sample by making a “dilution cuvette”. Sample is aspirated from the
dilution cuvette and dispensed into the testing cuvette. The reagent blank,
calibration and QC samples are also pre-diluted for any tests programmed to
pre-dilute. The result printed is correct for the programmed dilution.
Pre-dilution Rates: If the “Pre-dilution Rate” is set in the “Specific Test Parameters” screen,
sample pre-dilution is performed according to the dilution rate (5 to 100). For reference see the
dilution rate and sample volume required for dilution in the examples below:
sample pre-dilution rate sample Volume
( µ l)
sample dilution
(water, µ l)
total
5 48 192 240
10 24 216 240
50 5 245 250
100 2.5 248 250.5
The reagent probe dispenses liquid from the pre-dilution bottle into a “dilution” cuvette. Then
the sample probe dispenses sample into the dilution cuvette to make the set pre-dilution. The
sample probe then aspirates from the dilution cuvette into the test cuvette. Reagent blank,
calibrators, and QC samples are also pre-diluted for tests programmed to pre-dilute.
Min. OD An absorbance check for methods defined as FIXED, FIXED 1, RATE, or
RATE 1. The B flag is generated for reactions less than the minimum OD
Max. OD An absorbance check for methods defined as FIXED, FIXED 1, RATE, or Rate
1. The D flag is generated for reactions greater than the maximum OD
Reagent OD Limit Enter the acceptable ranges (L and H) for the Reagent Blank. The following
Flags are generated when the ranges are exceeded:
First L: u The reagent absorbance is below the programmed range at the
first read point.
Last L: U The reagent absorbance is below the programmed range at the
last read point.
First H: y The reagent absorbance is above the programmed range at the
first read point.
Last H: Y The reagent absorbance is above the programmed range at the
last read point.
Dynamic Range: Enter the linear range for the test. The flags are generated:
F : results are greater than the high end of the dynamic range.
G : results are less than the low end of the dynamic.
Correlation Factor The analyzer software is designed to perform the dynamic range check before
the correlation factors are applied to the data. Therefore, consider adjustment
of the dynamic range settings in accordance with the correlation factors.
y = Ax +B •
“A”: Multiplication factor that defaults to 1.0 •
“B”: Addition/Subtraction factor that defaults to 0.0 •
Adjusts data realtime when factors are programmed. •
Onboard Stability
Period
Enter 1 to 999 for the number of days a reagent is stable once it is placed on
the analyzer. Press the spacebar, then enter, if no setting is desired. The “a”
flag is generated when the onboard stability period is exceeded.