CONFOCOR
3
ConfoCor 3
Introduction to Laser Scanning Microscopy and Fluorescence …
Carl Zeiss
02/2010 M60-1-0025
e
7
For ConfoCor 3 operation, the mirrors will be parked.
Selection of the specimen detail of interest for zooming is fast and convenient, and the zoomed image is
automatically centered. This saves the job of specimen centration with the microscope stage.
Using a bi-directional scanning facility will double the scanning rate to approx. 5 frames/sec (at 512
×
512
pixels); if two different laser wavelengths are used for the two scanning directions (wavelength 1 for left-
to-right, and wavelength 2 for right-to-left scanning), two fluorochrome dyes can be viewed and
documented in a quasi-simultaneous mode. This will prevent cross talk between detection channels.
9.1.3.2
Microscope Equipment of the ConfoCor 3 and the LSM 710 and LSM 780 Systems
The LSM 710 and LSM 780 systems are equipped either with the inverted Axio Observer.Z1 SP
microscope required in combination with the ConfoCor 3.
Only the differences from the delivered operating manual "Axio Observer.Z1" will be explained here.
(1) Stand
a)
The motorized objective nosepiece 5
×
H DIC is firmly fixed to the stand, where no operating elements
can be found for the nosepiece. Operation will be performed via LSM software control. The "Restriction
of the nosepiece height to protect the objectives during motorized objective change" is inactivated. The
nosepiece will be moved down automatically before each motorized objective change.
b)
The reflector mount is motorized and provided with the Axio Observer.Z1 reflector turret. The reflector
turret has six positions: One transmitting light position, which is identical to the LSM position, and five
further positions for fluorescence filter sets (reflector modules). If you want to use more than five
conventional fluorescence filter sets, it is advisable to use a further reflector turret. When changing the
reflector turret position you must make sure that the turret will click into position, since otherwise the
image area will be cut.
c)
The stand has a motorized focusing drive (fine coarse). Sensitivity of the focusing drive is adjusted to
the delivered objectives by the manufacturer. If you want to use other objectives, sensitivity and
parfocality can be adjusted via the Axioset program.
d)
The stand features an integrated power supply for the internal motors and stand electronics. The
power supply can be switched at the external power supply unit for the microscope. External power
supply units will be used for the mercury vapor short arc lamp or the X-Cite 120 fiber coupled lamp.
e)
The analyzer slider for conventional DIC methods will be operated from the right side and is located
just below the nosepiece.
When the rod is pushed in, the analyzer is located in the beam path. In the LSM-mode, the analyzer must
not
be located in the beam path, and the analyzer rod must be pulled out.
(2)
Specimen stages and fine focus drives
a)
Mechanical stage
The stage with coaxial drive must be mounted on the right side of the stand.
b)
Scanning stage
c)
Piezo objective focus drive
