CONFOCOR
3
Carl Zeiss
Carrier Position
ConfoCor 3
16 M60-1-0025
e
02/2010
9.5.2
Z (Focus) Control
The functions of the
Z (Focus)
tool allows you to
position the focus in Z direction.
•
Press the
Z (Focus)
tool tab to open the
Z (Focus)
tool box (Fig. 13).
Press anywhere outside the window to close the
tool box.
If the LSM was used for scanning, light will
only be directed to the CCD camera once an
FCS application was triggered, even if laser
button is in the "on" position.
The following functions are available:
Laser
button (laser warning icon): Switches selected laser line illumination on the sample on / off.
Z-Position
display field: Displays the current Z position.
Store
button: Stores the current Z position.
Reuse
button: Moves the stage / nose piece to the stored Z position.
Work
button: Moves the stage / nosepiece back to the Work position. This is the position last set before
the
Load
button was pressed.
Load
button: Lowers the stage / nosepiece to a default position make it easier for you to change the
sample carrier (or objective).
+200 µm
button: Moves the Z drive for +200 µm per mouse click upwards.
-200 µm
button: Moves the Z drive for -200 µm per mouse click downwards.
The Z-position stored by activation of the
Store
button is a relative parameter and is only valid
during the current session. Accordingly, this position can be approached in a defined way only
during this session via
Reuse
.
If the sample is just a solution without structure, one cannot "focus" the instrument by looking at sample
features. Cover slip reflection has to be used instead to find the glass- / solution interface. The position of
the glass surfaces is detected most conveniently via line scanning.
Fig. 13
Z (Focus) panel
