LSM 710 and LSM 780
LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
Systems
Acquisition Tab
Carl Zeiss
02/2010 M60-1-0025
e
63
5.2.5.6
Ratio Imaging Configuration
In the
Show all
mode
of the
Light Path
tool
two
additional ratio imaging channels
R1
and
R2
can
be defined. These channels are displayed in
addition to the maximum of 8 active imaging
channels. They provide the possibility to display the
ratio between two of the active channels.
A click on the arrow next to
Ratio
in the lower
part of the
Light Path
tool window (Fig. 53)
provides access to the ratio channel interface. A maximum of two ratio channels can be activated. These
channels can display the ratio between two of the active imaging channels. The source channels for
calculating the ratio have to be selected from the drop down menu next to Source 1 and Source 2. As an
alternative to a second channel for the ratio imaging is also possible to choose the first images of a time
series of one channel for the ratio metric calculation (e.g. to calculate F/F
0
for single wavelength dyes). The
parameters for the calculation are set in the
Channels
tool (see section
Tool Group Online
Acquisition: Channels Tool
).
A suitable color is assigned to each of the two
Ratio Channels R1
and
R2
, in the same way as for the
photomultiplier channels.
The ratio to be formed between the selected channels can be defined more precisely using one
of the four preset formulas in the
Channels
tool
window after a click on the relevant ratio
button (e.g.: R1) for online display of radiometric or single wavelength dyes. The Set by min/max
function (in
Channels
tool window) allows the definition of the display scaling according to the
expected minimal and maximal values.
Fig. 53
Ratio channels in the Light Path tool
