CONFOCOR
3
ConfoCor 3
Options
Carl Zeiss
02/2010 M60-1-0025
e
129
•
With the overlay tool (Fig. 138) measure the
offsets in the x and y directions between the
bleached spots and the cross-lines (Fig. 139).
Enter the average values in the
LSM-ConfoCor
menu.
−
If the bleached point is left to the marker:
type distance in X as positive value
−
If the bleached point is right to the marker:
type distance in X as negative value
−
If the bleached point is below the marker:
type distance in Y as positive value
−
If the bleached point is right to the marker:
type distance in Y as negative value
−
Offset Z: no entry
Check the correctness of your settings by repeating
the adjustment procedure (Fig. 140).
If the deviations between bleached areas and
markers are marginal, the LSM / FCS superposition
alignment is sufficient to guarantee a precision of
1 µm.
Please note, that precision is higher if you
decide to make a measurement in one
section of the image rather than all over it. In
this case only deviations in that sector should
be taken into account for calculating the
offset values.
Fig. 138
Overlay tool
Fig. 139
Image of dye layer with overlay
Fig. 140
mage after offset correction
