CONFOCOR
3
ConfoCor 3
Action Buttons
Carl Zeiss
02/2010 M60-1-0025
e
115
Status lines at the bottom of the data
evaluation window and in the
Open Image
display area show the progress of a current
measuring procedure online. For non saved
modified data a warning sign (orange
triangle) will be displayed in
Open Images
(Fig. 118).
•
Click on the
Stop
button to end the
measurement. Only complete measurements
will be available for storage; the interrupted
measurement will be lost.
•
Save the data in the
File
menu.
(1) Positioning
in
solution
•
Align a carrier in the
Carrier Position
tool. Focus into the solution.
•
Go to the
Measure
tool and the
Acquisition
panel. Choose Current Position or Sample Carrier and
select the well(s) you want to take the measurement in.
(2)
Positioning in a cell
Before positioning you have to select appropriate cells and image those. An overview of fluorescent
expressing cells is best achieved by using the binoculars in transmitted and epi-fluorescent mode. Once
cells with adequate expression levels have been identified they can be imaged with the LSM.
Alternatively, suitable cells can be searched by using low zoom values in the LSM.
Visual inspection of the cells via binoculars
•
In the major toolbar activate the
Ocular
button
.
Fig. 118
Open Images with measurement in
progress
