CENTER SCREEN AREA / IMAGE CONTAINERS - …
LSM 710 and LSM 780
Carl Zeiss
Kinetic / FRAP View: Additional View Type …
Systems
228 M60-1-0025
e
02/2010
Example: FRAP Performed in a Nucleus Expressing GFP Labeled Proteins
Display of the image series in the
Mean ROI
display mode: The drawing tools are used to define the ROI
to be analyzed (ROI 1), the background ROI (ROI 2), and the reference ROI (ROI 3) (see Fig. 254). The
reference ROI must be a neighboring cell which has been imaged with the same laser intensity over time
identical to the cell, which has been bleached to induce FRAP. Make sure the whole cell or cell
compartment of interest is imaged and therefore illuminated.
Use the
time
slider in the
Dimensions
view options control block of the general view options
group to display the first image after the bleach event. This makes it easy to choose the ROI for
analysis. The analysis region should be slightly smaller than the region that has been bleached.
The latter is listed in the Mean of ROIs list in the overlay view options control block.
•
Define your ROIs for analysis in the
Mean of ROI
View tab.
Fig. 254
FRAP data displaying the first bleached image of an images series in the Mean of ROI View Type