LSM 710 and LSM 780
LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
Systems
Ocular Tab
Carl Zeiss
02/2010 M60-1-0025
e
47
(2)
Microscope settings on Axio Imager for reflected-light observation (Epi-fluorescence)
•
Turn on the HBO 100 W or X-Cite power supply with switch (Fig. 33/
8
)
•
Click on the
RL reflected light
button. The shutter opens.
To avoid excessive bleaching of biological samples, expose the specimen to the minimum
possible irradiation, i.e. keep the irradiation time as short as possible. For this, use the slider in
the Ocular panel to attenuate the illumination of the HBO / X-Cite.
•
By clicking on the reflector turret button, select the reflector module (filter sets) to suit the type of
fluorescence excitation. Proceed as follows:
•
Click on the reflector turret
button.
•
Click on the desired reflector module.
−
The reflector turret moves the selected reflector module into the beam path.
The FITC filter set consists of an excitation filter for the 450 - 490 nm spectral range, an FT color
splitter for 510 nm and an LP long pass filter, which passes emission light wavelengths greater
than 510 nm (FSET 09 = FITC, FSET 15 = Rhodamine, FSET 01 = DAPI).
Other filter sets:
DAPI:
BP 365 FSET01
FT
395
LP
397
Rhodamine: BP
546
FSET15
FT
580
LP
590
The filter sets described in this section are examples; other sets are available on request.
The aperture setting on the condenser of the Axio Imager.Z2 is performed in fixed steps.
