LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
100 M60-1-0025
e
02/2010
5.2.19
Image Optimization
This section describes an example how to acquire an image, using an excitation wavelength of 561 nm
and a fluorescence emission range above 570 nm. Use the MBS 488/561 as the main dichroic beam
splitter. We presume that no predefined configuration is available for this imaging task.
The description applies to the use of any microscope.
5.2.19.1
Requirements
•
Position the specimen under the objective and
focus onto the desired imaging plane.
•
Activate the main tool tab
Acquisition
and
open the
Imaging Setup
tool. Chose
Channel
Mode
for the image acquisition and check the
box next to Track1 (Fig. 95).
•
Switch on the Laser 561 nm.
•
Open the
Light Path
tool.
•
Click on the laser icon, select the 561 line, and
set the transmission slider to about 3 % (this
laser has an output power of 10 mW, other
lasers have up to 100 mW depending on the
type of laser and the imaging device used; be
careful when setting the power for imaging
with other lasers for imaging other dyes). Make
sure other lasers are not checked.
Optimize the laser intensity subsequently
via the
Transmission
slider when
imaging.
Fig. 95
Imaging Setup tool – imaging mode
selection and track activation
Fig. 96
Laser line and laser power selection
