CONFOCOR
3
ConfoCor 3
Models
Carl Zeiss
02/2010 M60-1-0025
e
71
Background
•
You can set an offset by setting a value in the
Background
selection box (Fig. 60). Either type
in a value or use the arrows.
The description box gives you information on the
background.
If there is no background selected, the back-
ground correction factor will be set to 1.
Amplitude
•
You can set a value for the geometric factor
γ
,
which describes the point-spread function
(Fig. 61).
•
By pressing the
cylindrical
,
2DG
,
3DG
and
GL
buttons you can select a cylindrical, 2
dimensional Gaussian, 3 dimensional Gaussian
(3DG) and Gaussian-Lorentzian (GL) PSF,
respectively. If you press the Calibrate button,
you can type in a user defined number in
selection box or use the arrows to set a value.
The description box gives you information on the
amplitude.
At least one the
γ
factor or the number of
molecules N have to be fixed in the fit
procedure.
Fig. 60
Background Settings panel
Fig. 61
Amplitude Settings panel
