LSM 710 and LSM 780
LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
Systems
Acquisition Tab
Carl Zeiss
02/2010 M60-1-0025
e
61
5.2.5.1
Activation/Deactivation of Channels
•
To activate a channel for imaging, check the check box
next to channel icon. To deactivate the channel uncheck
this box.
•
Use Channel D (transmission channel) to acquire an
image with transmission illumination using any laser for
excitation. This image will display the differential
interference contrast (DIC) of the specimen if the
according optical hardware for this contrast is put into
the beam path in front of the detector. Check the
manual of the microscope for setting DIC optics. Be
aware that the laser light is already polarized and
therefore a polarizer device is not necessary for
transmission imaging. This imaging mode allows
displaying a DIC image simultaneously to the
fluorescence signal of the specimen.
•
To assign a specific color to the image of a channel click
onto the color icon and select a color from the drop
down menu (Fig. 51).
5.2.5.2
Reflector Turret
The filter wheel FW1 is the reflector turret of the microscope that holds push-and-click filter cubes and
fixed beam combiner cubes. When using the LSM 710 and LSM 780 systems for imaging the filter wheel
is automatically set to an empty position if not otherwise defined or set by loading an imaging
configuration. Standard fluorescence filter cubes are used for observation of the specimen with the eye
or with the camera. A click on the icon opens a drop down menu from which the desired filter can be
chosen.
5.2.5.3
Stage / Focus
This icons provide access to the control for the focus (position of the specimen in Z) and the stage
(position of the specimen in X and Y). But detailed description is given in section
Tool Group Online
Acquisition: Focus Tool
and section
Tool Group Online Acquisition: Stage Tool
.
Fig. 51
Drop down menu for
selection of the channel color
