CONFOCOR
3
Carl Zeiss
Acquisition
ConfoCor 3
36 M60-1-0025
e
02/2010
Repeat Count
The
Repeat Count
value determines the number of measurements, i.e. how often
Measure Time
is to
be performed in a row, or in other words the repetitions. Enter a number in the input box or use the
scroll arrows.
Note that repetitions can be averaged into an average correlation function if required. This will
improve statistics. An average of 10 x 10 seconds measurement is not but nearly as good as one
100 sec measurement. If bleaching becomes a problem, it might help to reduce measurement time
and increase repetitions to obtain nearly as good statistical results while reducing bleaching.
Procedure for setting the times for an experiment:
•
Set the
Bleach Time
.
Start with the
Bleach Time
of 0 s. If the signal decreases during the subsequent measurement, this
might be caused by bleaching. As a remedy, you can pre-bleach the sample before measurement by
setting the
Bleach Time
to a non-zero value. If no
laser
check box was activated, no bleaching
occurs, but the measurement will be delayed for the bleach time.
•
Set the
Measure Time
.
If you do not have any idea about the behavior of your sample, start with a measurement time of
about 10 seconds. Depending on the signal-to-noise ratio of your correlation curve, decrease or
increase the measurement time.
•
Set the
Repeat Count
.
Start with
Repeat Count
set to 1. If you want to obtain information about the variation of your fitted
values later on, increase the repeat count to produce a measurement series. 10 times is a good value
to start with.
The total duration of the measurement will be indicated.
