LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Maintain Tab
Systems
144 M60-1-0025
e
02/2010
5.4.1.3
How to Adjust the Pinhole
Adjustment of pinholes can be performed
manually or automatically.
Temporary optimization of the adjustment:
The position of the pinhole relative to the detector
in terms of X-Y-Z coordinates contributes
substantially to image optimization.
Requirements to make the influence of pinhole
position changes on image brightness immediately
visible:
−
The image must be scanned by the
continuous scan method.
−
Select a fast scanning speed.
−
Measurement with Average Number 1 only
(no averaging of several measurements).
−
On the
Channels
tool in the LSM
Online
Acquisition
tool group select the pinhole
diameter so as to have the best possible
image contrast (see Fig. 155).
•
Open the
Adjust Pinhole and Collimator
tool
in the
Maintain
tool group in the
Maintain
panel.
•
Select the pinhole to be adjusted from the
Name
list box.
•
Use the
Diameter
slider to set the smallest
possible size which produces a good, high-
contrast image.
−
This setting changes the pinhole diameter.
−
The
Optimal Interval
box of the
Optimize
Sectioning and Step
submenu of the
Z-Stack
tool in
Show all
mode within the
Multidimensional Acquisition
tool group
simultaneously displays the depth resolution
corresponding to the pinhole diameter (see
Fig. 156).
Image optimization can be effected with
the
Range Indicator
or in the
Line-Scan
mode.
Fig. 155
Channels tool
Fig. 156
Z-Stack tool
