LSM 710 and LSM 780
MULTIPHOTON LASER SCANNING MICROSCOPY …
Systems
Non Descanned Detection (NDD)
Carl Zeiss
02/2010 M60-1-0025
e
5
8.2
Non Descanned Detection (NDD)
The use of Non-Descanned Detectors with the
LSM 710, LSM 780 NLO and LSM 7
MP
is only
useful in combination with the NLO laser.
The Non-Descanned Detection modules can be
used on the reflected or transmitted-light beam
path (transmission NDDs not available for Axio
Imager) or simultaneously on both beam paths.
This means that up to 10 NDD channels
(depending on the microscope used) can be
configured. If NDD channels have been assigned to
the transmitted-light beam path on Axio Observer,
no transmission PMT can be implemented.
In Non-Descanned Detection, the radiation emitted
by the specimen is directly guided onto the
relevant detector without passing the scanner
mirror again.
Non-Descanned Detection is set and configured in
the
Light Path
tool by choosing the
Non-
Descanned
tab next to the
LSM
tab. This tab is
only available when NDD detectors are connected.
•
Click on the
Non-Descanned
tab to change to
Non-Descanned Detection.
•
After checking a box next to a channel the
corresponding detection window is indicated in
the bar in the upper part of the window. Within
each bar an arrow opens a drop down window
which allows choosing the fluorescent dye to be
imaged. Its emission profile is then indicated in
the spectral display.
The configuration of multiple tracks is
possible for NDD applications, but they can
not be combined with tracks which are set
up with the standard channels.
Fig. 2
Light Path tool with NDD detectors
(3 in reflection, 3 in transmission)
plus GaAsP nosepiece NDD detector
on Axio Examiner
