By connecting adjacent detector elements (bin-
ning), the spectral width of the images can be
extended. From a lambda stack, the intensity of
the signal for each pixel of the image can be
extracted as a function of wavelength. These
spectral “fingerprints” can easily be obtained for
any image area by means of the Mean of
ROI
function. Lambda stacks can be recorded as time
series, Z stacks, or as Z stacks versus time. In the
last-named case, the result would be a five-dimen-
sional image file with the coordinates, X, Y, Z,
lambda and time.
The META Detector is good not only for recording
lambda stacks, but also as a channel detector in
the conventional mode. By binning the respective
detector elements in this mode, the optimum
spectral bandwidth can be adjusted for any fluo-
rescent dye.
15
Y
X
λ
Lambda stack
All images show the same area, but different
spectral windows of the specimen.
The marker dyes are represented by different parts
of the stack depending on the emission spectrum.
