LSM 710 and LSM 780
CENTER SCREEN AREA / IMAGE CONTAINERS - …
Systems
Polarization Imaging
Carl Zeiss
02/2010 M60-1-0025
e
269
In the
Ratio1
channel choose the
Ratio Type 3
formula. With Ch1-T1 and Ch1-T2 corresponding to I
P
and Is this formula corresponds to the formula for anisotropy r. For the factors put in the value for the G-
factor in the numerator and 2 x G in the denominator. Scale by Min/Max by checking the
Set by
min/max
box and entering
0
in the
Min input box
and
1
in the
Max input box
. With a corresponding
look up table like rainbow this will produce a fasle colored anisotropy image in the nRatio1 channel.
Alternatively you can use the image calculator with source 1 (S1) being the
P
track image (Ch1-T1) and
source 2 (S2) the
S
track image (Ch1-T2) with consideration of the G-factor (see Fig. 322).
Proceed as follows. In the
Processing
tab select the
Image calculator
. Press
Select
and click on the
desired input image. In the
Channel
pull down menu of
Input Image Image 1
select
ChX-T1
, where X
stand for 1, 2 or S. Accordingly, in the
Channel
pull down menu of
Input Image Image 2
select
ChX-T2
.
Type in the anisotropy formula considering the G-factor of the system. Choose either Absolute Intensities
in case you have to multiply the formula with 2
n
-1 to rescale to an n bit image. For example, if the color
depth was 8 bit, multiply with 2
8
-1=256-1=255. Alternatively, choose
Intensity range 0..1
, in which
case you do not multiply with an extra factor. Press
Apply
and choose a respective look up table to
obtain a false colored anisotropy image.
