12
02/2010
Configuring the beam path and lasers
•
Click the
Acquisition
button.
Smart Setup
The tool
Smart Setup
is an intuitive, user-friendly interface which can be used for almost all standard
applications. It configures all the system hardware for a chosen set of dyes.
•
Click on the
Smart Setup
button
to open the smart setup window. This window can
be accessed any time from the software to change dye combinations.
•
Click on the arrow in the dye list and simply choose the dye(s) you want to use in your experiment
from the list dialogue. In this dialogue, the dyes can be also searched by typing the name in the search
field.
Fig. 14
Smart setup tool
Once finished with the input,
Smart Setup
suggests four alternative considerations (see below): One for
fastest
imaging, one for the
best signal
,
best compromise
between both speed and best signal and
the optimal setup for later
linear unmixing
of the dyes.
The graphs display relative values for the expected emission signals and cross-talk. The resulting imaging
scheme (single or multitrack) is shown below the graphs.
