LSM 710 and LSM 780
MULTIPHOTON LASER SCANNING MICROSCOPY …
Systems
Non Descanned Detection (NDD)
Carl Zeiss
02/2010 M60-1-0025
e
9
All emission filters offered for the NDD modules have an additional IR blocking capacity of OD 5. Other
filters without these characteristics may lead to inferior image quality.
All secondary dichroics for the NDDs Push&Click filter cubes are long pass filters. They split the emitted
fluorescence according to the indicated wavelength into the longer and shorter spectral range. In this
respect the dichroics are specified in the spectral range of 380 to 800 nm.
The Emission filters are mounted into Push&Click filter cubes which are clamped into the slider of the
NDD modules. NDD modules with two channels hold two Push&Click filter cubes. NDD modules with one
channel hold one Push&Click filter cube. The NDD module to be used for the end of the cascade does
not have a slider for a Push&Click filter cube.
•
To change the cubes simply take out the sliders which hold the cubes by unscrewing them from the
rest of the module using the thumbscrews (Fig. 7/
1
).
•
The filter cubes can now be mounted onto the slider by sliding the side noses of the cubes under the
corresponding spring latches. The orientation of the dichroics and emission filters is indicated on the
slider.
The arrangement of the filters inside the NDD modules depends on the orientation of the modules on the
microscope.
For LSM 710 NLO and LSM 780 NLO systems on
Axio Observer
the NDDs are attached to the sideport
(up to 5 channels are possible) or the transmission port (maximum of two channels is possible). All
dichroics offered by Zeiss for this modules, are long pass dichroics. Therefore the blue part of the
spectrum is detected with the first NDD whereas the red part is guided further on to the following NDDs
(Fig. 8).
Fig. 8
Example for a filter equipment of a four channel NDD set up on Axio Observer starting with
an NDD module type C or D
For LSM 710 NLO and LSM 780 NLO systems on
Axio Imager
(NDDs are only available for reflection) and
Axio Examiner
a total of 5 detectors can be mounted onto the ports starting of with a two channel
module of type A or B.
The filter arrangement could be as described in Fig. 9. As the dichroics are also long pass filters channel 1
always needs to see the red part of the spectrum followed by the blue part of the spectrum within the
required detection range. If more than 4 channels are required in reflection, a fiber coupled illumination
system (X-Cite 120) instead of a standard HBO lamp is required.
