LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Processing Tab
Systems
132 M60-1-0025
e
02/2010
As the resolution of an optical system is
significantly lower in the axial direction than in the
lateral (X/Y-)direction, the greatest improvement in
resolution can be achieved in the Z-direction.
The Z-Stack must meet the following requirements:
−
At least two-fold oversampling in xyz (z: half of
optimal interval button)
−
High signal-to-noise ratio
−
Detector gain < 500 V
Calculation is either made for one channel of the
opened image which must first be selected
accordingly, or for all channels of a stack.
Calculation is started via
Apply
and can be
stopped using the
ESC
key, if required.
Fig. 141
Image of a fluorescent bead with a
diameter of 1µm before deconvolution
(A,B) and after deconvolution (C,D)
