MULTIPHOTON LASER SCANNING MICROSCOPY …
LSM 710 and LSM 780
Carl Zeiss
Non Descanned Detection (NDD)
Systems
8 M60-1-0025
e
02/2010
Fig. 6
Power connections of NDD detectors and the scan modules on the electronics rack of the
LSM 710 NLO and LSM 780 NLO.
8.2.3
Filter for non descanned detectors
(NDDs)
To make the best use of NDDs it is necessary to
achieve optimal blocking of the excitation laser
light. This is done in the same way for all
LSM 710 NLO and LSM 780 NLO systems with
NDDs independent of the microscope used.
The first NDD module, which is attached to the
NDD port, is always a two channel module
(Fig. 7/
1
). Inside this module right at the opening
towards the microscope is a small clamping that
holds a blocking filter (1442-160 or 1442-161;
order# Feb. 08). Both filters are short pass filters
which block the IR light of the multiphoton laser
with an optical density (OD) of at least 7. If none
of these filters is installed, the image quality will
not be sufficient as too much laser light will get to
the detectors. Always make sure on of these filters
is in place.
To install them, open the metallic grey lid of the NDD module turning the thumbscrews (Fig. 7/
1
). Once
the lid is removed the clamping is accessible. Slid in the filter, which is attached to a small holder, into the
clamping with the little screw on the side of the holder situated on the left side of the holder when
facing towards the microscope.
Fig. 7
Thumbscrews of the P&C sliders
