02/2010
1
Contents
Page
Contents ................................................................................................................................. 1
Introduction............................................................................................................................ 1
Starting the System ............................................................................................................... 2
Introduction to ZEN – Efficient Navigation .......................................................................... 5
Setting up the microscope................................................................................................... 10
Configuring the beam path and lasers ............................................................................... 12
Scanning an image............................................................................................................... 17
Storing and exporting image data ..................................................................................... 22
Using the ConfoCor 3 module............................................................................................. 23
Switching off the system..................................................................................................... 29
Introduction
This Quick Guide describes the basic operation of the LSM 710, LSM 780, LSM 710 NLO, LSM 780 NLO
and ConfoCor 3 Laser Scanning microscopes with the ZEN 2010 software.
The purpose of this document is to guide the user to get started with the system as quick as possible in
order to obtain some first images from his samples.
This Quick Guide does NOT replace the detailed information available in the full user manual or in the
manual of the respective microscopes (Axio Imager, Axio Observer, Axio Examiner).
Also, this Quick Guide is written for a user who is familiar with the basics of Laser Scanning Microscopy.
For your safety!
Observe the following instructions:
−
The LSM 710, LSM 780, LSM 710 NLO, LSM 780 NLO and ConfoCor 3 Laser
Scanning Microscope, including its original accessories and compatible accessories
from other manufacturers, may only be used for the purposes and microscopy
techniques described in this manual (intended use).
−
In the Operating Manual, read the chapter
Safety Instructions
carefully before
starting operation.
−
Follow the safety instructions described in the operating manual of the microscope
and X-Cite 120 lamp / HBO 100 mercury lamp.