CONFOCOR
3
ConfoCor 3
Processing
Carl Zeiss
02/2010 M60-1-0025
e
47
The X-, Y- and Z- coordinates of the selected positions are only stored in the
Positions
table and
not in the scanned image (stack). Accordingly, all the marked positions in all the Z-planes are
displayed in the scanned image (Z-stack), no matter in which Z-plane they lie.
•
When finished, click on the
Snap
or
Start ConfoCor Experiment
action button to start the FCS
measurements at the highlighted or all listed positions, respectively. The measurement procedure
starts and the
Image FCS diagram
window appear in a container.
Please note that the positions selected for FCS measurements in the
Measurement
window using
the ConfoCor 3 are approached by the scanning stage, Piezo stage or scanning mirrors, whichever
was chosen. In the ConfoCor 2 scanning mirrors are not available for positioning. All positions will
be approached sequentially.
In using the scanning mirrors there is no offset between defined positions and actual positions.
However, a scan correction in x and y can be required due to scanner hysteresis, which is done by
changing the
Scan Corr X
and
Scan Corr Y
positions in the Scan window, if the bidirectional scan
was chosen. If stages are used, the offsets can be entered in the
Settings
menu under
LSM +
ConfoCor
.
9.8
Processing
In the Process panel you can define the fit and
acquisition parameters for the measurement.
•
Click on the
Processing
tab to open the
processing control panel (Fig. 50). Click on any
other tab to leave the panel.
9.8.1
Fit
The
Fit
panel of the menu allows you to load in a
fit model and define how the parameters are to be
fitted.
•
Click on the
Fit
tab to open the
Fit
control
panel (Fig. 51). Click the tab again to close the
panel.
•
Select a channel by pressing the
Ch1
,
Ch2
or
Cross Correlation
tabs, if applicable. Note that
channels will only be active if selected in the
current configuration. To each channel a
different Fit model can be assigned.
Fig. 50
Processing panel
Fig. 51
Fit panel
