LSM 710 and LSM 780
ANNEX
Systems
Beam path Configuration Guide for …
Carl Zeiss
02/2010 M60-1-0025
e
7
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Select the proper emission bands in front of the channels and activate channels.
Example: select > 575 nm in front of Ch 3, and a band of 500 – 550 nm in front of Ch 2.
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Make sure that the active detection bands do not include any of the active laser lines.
Additional hints:
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Do not forget to turn on the corresponding lasers and detectors.
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Assign appropriate colors to these activated channels. Example: Ch 2 - green (for Alexa 488 emission,
Ch 3 - red (for CY3 emission)
Fig. 1
Light Path
tool,
Imaging Setup
tool
