ANNEX
LSM 710 and LSM 780
Carl Zeiss
Beam path Configuration Guide for …
Systems
6 M60-1-0025
e
02/2010
7.3
Beam path Configuration Guide for Fluorescence Microscopy with the
LSM 710 and LSM 780 Systems
7.3.1
Optical elements in the Configuration Control window
All wavelength values given in Nanometer [nm].
7.3.1.1
Main Dichroic Beam Splitter (MBS)
•
A
MBS XXX/YYY
deflects the indicated laser lines onto the specimen and allows the emitted
fluorescent light to pass through.
Example: MBS 458/514, MBS 488/543/633 (deflects also UV excitation light)
•
A
MBS XXX+
is a special type of a main dichroic used for IR multiphoton excitation. The
MBS 690+
for example, deflects laser light with a wavelength longer as 690 nm onto the specimen and allows
fluorescent emission light in the visible range below 660 nm to pass through.
Example:
MBS 690+
•
A
MBS XXX/YYY+
is a combination of a MBS XXX and MBS YYY+ used for simultaneous IR multi
photon and single photon excitation.
Example: MBS 405/710+.
7.3.2
Setup of Tracks Using Single Detectors (channel mode)
a) One track only – simultaneous acquisition of channels
•
Switch on the suitable lasers for excitation of the dyes in the specimen. For the Argon laser set the
tube current of the laser to a value of app. 50% (
Show All
, as described in chapter 4, section 4.3 of
this manual). Example: for Alexa 488 and CY 3 switch on
Argon
(blue excitation) and
HeNe1 or
DPSS 561
(green excitation).
•
Activate the proper laser lines in the
Line Active
check box, set
Transmission [%]
for each active
line.
Example: Select 488 to 4% and 543 to 50%
•
Select a main dichroic beamsplitter (MBS) which deflects the selected laser lines to the specimen.
Example: MBS 488/543
