CONFOCOR
3
Carl Zeiss
Action Buttons
ConfoCor 3
120 M60-1-0025
e
02/2010
•
Open in the
Online Acquisition
tool group the
Acquisition Mode
tool (Fig. 125). Here you
can select the objective, scan modes, frame size,
speed, averaging conditions and the zoom.
•
Open in the
Online Acquisition
tool group the
Channels tool (Fig. 126) and set the pinhole
diameter (1 Airy Unit recommended), gain and
amplifier offset. Select the required laser line
and set the AOTF transmission. You can pres
the Find button to let the system optimize on
the required gain.
•
Press the LSM
Scan
action button to start a
continuous scan. Optimize the laser settings
while observing the image. If required refocus
and reposition your sample.
•
Either manually position the site of interest
using
Current
in the
Measurement
tool’s
Position
menu, or approach defined positions
automatically in
LSM Image
.
Fig. 125
LSM Acquisition Mode tool
Fig. 126
LSM Channels tool
