LSM 710 and LSM 780
LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
Systems
Acquisition Tab
Carl Zeiss
02/2010 M60-1-0025
e
85
A region drawn in the image will be listed in the regions tool. The regions are assigned with numbers. By
checking the box next to
Hide
the regions will not be shown in the image. When highlighting a ROI in
the list, the numerical parameters for
Center X
,
Center Y
,
Width
and
Height
will be shown below. In
addition the ROI will be set to active in the image.
The regions can be used for different tasks. By checking the appropriate box the ROIs can be used for
acquisition, bleaching, and analysis. Any combination of these tasks is possible.
It is possible to change the
Line width
and the
Color
of the outline of the region. The latter is only
possible if either
Individual Color
or
Common Color
is chosen in the drop down list of the
Color
Mode
.
Common Color
assigns the same color to all regions. If
Automatic Assignment
is chosen in the
Color Mode a color will be automatically assigned to each region as it comes. The choice of Color is
inactive in this mode.
The
Creation Mode
offers three different settings for defining ROIs.
Switch to selection mode
activates the arrow tool once the region is drawn in the image.
Multiple elements
allow drawing
multiple regions of the same type in a sequence.
One element only
restricts the number of ROIs to be
drawn in the image to one. Any new region deletes the previous one.
The laser usually scans the entire line length, but is limited in the Y-direction by the ROIs outline. The
Scan Time is therefore reduced. It is possible to reduce the movement of the scanner also in the X
direction along the line by checking the box next to
Fit frame size to bounding rectangle of regions
.
This then corresponds to zooming in onto the region. The frame size will be updated accordingly in
addition to the scan time. This applies for image acquisition using a ROI.
If a region is used to define a position for bleaching within the sample, the bleach process can be
accelerated by checking the box next to
Zoom Bleach (fast, less accurate)
. In this case the scanner
movement will be restricted to the bleach region zooming in onto this region. This may result in a less
accurate positioning of the region as the definition of the region has been made in a different zoom in
the image. The bleach process will be faster than without this option. The gain in speed is dependent on
the speed that is used for bleaching. If a high speed has already been chosen for bleaching the gain in
speed will be minimal.
The numbers of the different regions can be shown in the image if the check box next to
Show
numbers
is checked.
The regions can be saved to be loaded later on. This process stores all regions currently displayed in the
Regions tool in one file.
