CONFOCOR
3
Carl Zeiss
Acquisition
ConfoCor 3
44 M60-1-0025
e
02/2010
You can also use the arrow buttons or the joy stick to move the microscope stage, but in those cases,
the orientation gets lost. The selected chamber will not any more be highlighted in blue in these case.
You can set the size of the steps to be performed via the
Step Size (µm)
scroll menu (or the input
box) and the speed in the
Speed Settings
drop down menu.
Regardless which well is selected, in the result table of the
FCS results
window the position is
indicated as
"
1
"
.
The measurement will be triggered by pressing either the
Snap
or the
Start ConfoCor
Experiment
action button.
Multiple positions
•
Select from the
Working Direction
drop down menu an entry other than
Single position
.
•
Select as many individual well positions as required (all are selectable) by just clicking on them.
Alternatively, a whole block of chambers can be selected / deselected by drawing a frame which
contains the centers of the selected or deselected chambers.
By pressing the
Select All
button, all wells will be selected.
By pressing the
Clear All
button, all wells will be deselected.
Selected wells will be highlighted and will be approached sequentially as follows:
Row by row:
Measurement is performed row by row. Each row starts from the left site.
Row by row meander:
Measurement is performed row by row. Rows start alternatively from the left
and right. First row starts from the left.
Column by column:
Measurement is performed column by column. Each column starts from the top.
Column by column meander:
Measurement is performed column by column. Columns start
alternatively from the top and bottom. First column starts from the top.
If the
Snap
action button is pressed, only the first highlighted position will be approached. To
approach all listed positions sequentially the
Start ConfoCor Experiment
action button must be
pressed.
