LSM 710 and LSM 780
HARDWARE ASPECTS
Systems
Optical Diagram of the LSM 710 and LSM 780 Systems (Schematic)
Carl Zeiss
02/2010 M60-1-0025
e
9
2
Hardware Aspects
2.1
Optical Diagram of the LSM 710 and LSM 780 Systems (Schematic)
HAL Halogen
Lamp
HBO Mercury Vapor Short-Arc Lamp
MBS Main Beam Splitter
NDD Non-Descanned
Detector
PMT Photomultiplier
T-PMT Transmission-Photomultiplier
Fig. 1
Optical path, schematic (34-channel configuration)
The diagram above is a schematic representation of the LSM system.
Laser light is focused onto the specimen through an objective in a diffraction-limited mode. Light emitted
at the focal plane and at planes below and above it is directed via an XY scanner onto a main beam
splitter (MBS), which separates the emissions from the excitation light. The fluorescence signals are
separated from each other by the beam guides and directed to individual photomultipliers.
