CONFOCOR
3
Carl Zeiss
System Configuration
ConfoCor 3
26 M60-1-0025
e
02/2010
Emission filter 1 and 2 (EF1 and EF2):
The EF is used to narrow the fluorescence spec-
trum. The bandpass (BP) filter allows the range
within the specified border wavelengths to pass
through. The longpass (LP) filter allows the range
above the specified border wavelength to pass
through. The short pass (KP) filter allows the range
below the specified border wavelength to pass
through. The EFs are positioned in a 90° angle to
the incident beam.
•
Click on the EF2 or EF1 (Fig. 26) icon to open a
list of available emission filters.
All filters can be viewed by using the scroll tab.
•
Select the appropriate one by a mouse click.
•
Close the list by clicking the icon again.
Selection of channels
You can select channels by activating the
Channel
check boxes:
−
AC Ch1 and AC Ch2 are physical channels
for auto-correlation
−
CC Ch2
→
Ch1 and CC Ch1
→
Ch2 are
software channels for cross-correlation,
whereby AC Ch2 is shifted versus AC Ch 1
and vice versa, respectively
You can enter a special comment (for example the
used dye or a memorable name) into the
AC
Ch1
/
AC Ch2
input boxes.
Fig. 27
System Configuration tab - Light
Path panel with EF1 filter list
