LSM 710 and LSM 780
LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
Systems
Processing Tab
Carl Zeiss
02/2010 M60-1-0025
e
137
5.3.14
Adjust
5.3.14.1
Burn in Brightness and Contrast
The
Burn in brightness and
contrast
function
creates a new image document in which the
current brightness and contrast settings from the
Display View Options
control block (see section
Center Screen Area / Image Containers -
Display and Image Analysis
) are permanently
written to the image file (Fig. 148).
The
button selects the active image in the
Image Display
as the Input Image to be
processed.
•
After adjusting the brightness and contrast in
the
Display View Options
control block, click
Apply
to create the new image document with
the burnt in contrast and brightness settings.
5.3.14.2
Interpolate Brightness and
Contrast
This function permits a continuous contrast and
brightness adjustment in a Z-Stack or Z-Stacks over
time by interpolation between the starting and end
values of the respective image series. This permits a
post-acquisition compensation of signal loss in, for
example, thick tissue imaging where excitation and
detection efficiency decrease significantly.
Interpolation can be defined for the entire image
or only for individual channels. In addition, a set of
coordinates for which intensities are kept fixed
during the interpolation calculation can be defined
(Fig. 149).
The
button selects the active image in the
Image Display
as the Input Image to be
processed.
Fig. 148
Burn in Brightness and contrast tool
Fig. 149
Interpolate brightness and contrast tool
