LSM 710 and LSM 780
MACROS AND VISUAL BASIC
Systems Macros
Carl
Zeiss
02/2010 M60-1-0025
e
21
•
Choose split image for the image window display. You will see the scanned areas of both scanning
systems and an overlay.
•
Choose LSM (710) or
DuoScan
to adjust the image match.
•
While scanning, move the sliders of the Image match macro to get a perfect overlay of the scanning
and/or manipulation system relative to each other.
•
Offset X/Y adjusts the horizontal and vertical image shift
•
Amplitude adjusts a size difference between the images
•
Rotation compensates for a tilt in one of the images.
Store the final image, and use the ReUse function for future image matching. The Multitrack
setup and the Zoom factors will then be set automatically.
•
For LSM 7
MP
with two scanners, focus onto the grid using the imaging scanner (chose “LSM” in the
Macro window) and position the center of the grid sample into the center of the image. To do this
project the virtual grid into the image by checking the box next to
Test grid
in the macro window
(Fig. 21) and try to overlay the grid sample with the Test grid in the image moving the sample stage
accordingly. Fine adjust the image of the grid sample to the Test grid using the buttons for Offset,
Amplitude and Rotation.
•
Next switch to “DuoScan” and adjust the image of the grid sample to the Test grid using Offset,
Amplitude and Rotation. Do not move the sample grid during this step. Take care to use moderate
laser powers of only a few % as the Multiphotonlaser can destroy the grid sample.
Fig. 22
Split image
