LEFT TOOL AREA AND HARDWARE CONTROL TOOLS
LSM 710 and LSM 780
Carl Zeiss
Acquisition Tab
Systems
104 M60-1-0025
e
02/2010
•
To adjust the black level (background), use the
Digital Offset
slider so that areas without
picture content just show a trace of blue.
•
If necessary, re-amplify brightness with the
Digital Gain
slider.
•
Stop scanning.
•
Save the imaging configuration again as this
time it will also save the correct settings for the
detector, the laser, and the pinhole.
The signal-to-noise ratio can be substantially
improved by reducing the scan speed to an
acceptable level and averaging over several scans
(i.e. with an average
Number
greater than 1 for
the
Mean
average
Method
in the
Scan Control
window).
•
Use the
Speed
slider in the
Acquisition Mode
tool set the slowest acceptable scan speed.
•
In the
Number
text box of the
Averaging
panel enter the number of images to be
averaged.
Image optimization can be achieved faster if you select a smaller frame, since less data have to
be processed.
The greater the number of averages selected using
Mean
as average
Method
, the better the
image quality will be; the scan time is prolonged accordingly.
A similar procedure applies for image optimization if more than one channel is used and simultaneous
image acquisition is performed.
Make sure the suitable lasers are switched on and the channels are activated.
Pay attention to address the right channel when pinhole size, detector gain, and amplifier offset are
controlled as these are set individually for each channel.
Use the split view (see section
Split View
) to see the effect of the controls onto each channel separately.
If the acquisition is performed sequentially in with multiple tracks it is faster to activate only one track at a
time for image optimization.
.
Fig. 103
Image displaying good adjustment of
gain and offset
